Skip to Main Content
Pitt Home
Find People
School of Medicine
Toggle navigation
Skip Navigation Links
Home
About
Leadership
Mission
Department Statistics
Building Information
Thomas E. Starzl BST
Biomedical Science Tower 3
Directory
Primary Faculty
Secondary Faculty
Graduate Students
Fellows
Administrative Staff
Research Staff
Adjunct Faculty
Department Alumni
Research
Research Areas
Labs
Equipment
Funding Opportunities
Faculty Development Program
Responsible Conduct of Research
Shops
Cell Biology/Pharmacology Machine Shop
Education
Molecular Pharmacology Graduate Program
Current Students
Current Student Roster
Student Publications
Diversity, Accessibility, and Counseling
Student Awards
MPGP News
Prospective Students
Welcome from the Director
Research Opportunities
PhD Track
MD/PhD Track
Financial Aid
Graduate Faculty
Graduate Courses
Grad Student Lab Rotations
Grad Student Events
Grad Student Event Calendar
Graduate Student Event Schedule
Alumni/Career Development
Alumni
Alumni Contacts
Molecular Pharmacology Facebook Group
Molecular Pharmacology Student-Alumni Network (LinkedIn)
SOM Graduate Studies - Career and Professional Development
Executive Committee
Student Testimonials
IBGP
Grad Student Funding Opportunities
NIH T32 Training Grant
Outcomes
Predoctoral Fellowship
Admissions
Student Handbook & General Timeline
Links
Useful Links
School of Medicine
PubMed
Remote Resources
Biomedical Masters Program
Undergraduate Research
Summer Fellowships
Lab Rotations
Graduate Fellowships
News
Events
Calendar
Seminar Series
Links
Contact
Opportunities
Department
PORtal
Computer Resources Access Request
IT-Equipment-Facilities Support Request
Poster Requests
Reservation Calendars
Badge Access Request
Home
>
Directory
>
Primary Faculty
>
Sruti Shiva, PhD
Sruti Shiva, PhD
Professor & Vice Chair for Academics and Equity Associate Director, Vascular Medicine Institute
E1240 Thomas E. Starzl Biomedical Science Tower
200 Lothrop Street, Pittsburgh, , PA 15213
sss43@pitt.edu
Phone: 412-383-5854
Education
BS (Biomedical Sciences and Chemistry), University of South Alabama, 1999
PhD (Cellular and Molecular Pathology), University of Alabama at Birmingham, 2004
Links
PubMed
NIH RePORTer
Research Details
Publications
Awards, Honors, and Sponsored Research
Labs
Shiva Lab
Research Areas
Pharmacology of Cell and Organ Systems
Redox Pharmacology
Signal Transduction
Structural Pharmacology
Link
: Translational Program Project / Vascular Sub-Phenotypes of Lung Disease
http://www.vmi.pitt.edu/tPPG.html
Dr. Shiva’s research focuses broadly on understanding the mechanisms by which mitochondrial function is regulated, particularly by reactive oxygen and nitrogen species and the contribution of these mechanisms to cardiovascular health and disease pathogenesis. Using a wide spectrum of techniques ranging from the biochemical study of isolated mitochondria to whole animal models and measurement of bioenergetic function in human blood cells, the Shiva lab is currently engaged in a number of active projects along four major scientific themes:
1 – Utilization of platelets to measure human mitochondrial function in health and disease.
Mitochondrial dysfunction plays a role in the pathogenesis of numerous diseases, however data on human mitochondrial function is lacking due to the lack of non-invasive methodology to obtain sufficient quantities of live mitochondria. Platelets contain fully functional mitochondria and we have optimized and validated methodology (utilizing Seahorse XF analysis) to measure human platelet bioenergetics. We have used this technique to show that platelets from different patient cohorts have differing bioenergetic profiles. We are currently comparing platelet bioenergetics in a number of different patient populations to determine whether platelet bioenergetics can be utilized as a biomarker of disease and a measure of disease progression.
2 – The role of platelet mitochondria in hemolytic disease pathogenesis.
Sickle cell disease is characterized by severe hemolysis, which has been linked to platelet activation. We recently showed that hemolytic components directly inhibit mitochondrial oxidative phosphorylation leading to the production of oxidant which stimulates platelet activation. Current work in the lab aims to determine the role of this hemolysis-dependent altered mitochondrial function in sickle cell disease as well as expand these studies to other diseases with a component of hemolysis, including sepsis and pre-eclampsia.
3- The mitochondrion as a physiological target for nitrite.
Nitrite (NO
2
-
) is a signaling molecule that mediates a number of biological actions including protection after ischemia/reperfusion, reversal of symptoms of the metabolic syndrome and increased exercise efficiency. However, the molecular mechanisms underlying these actions are unknown. Dr. Shiva’s work demonstrates that nitrite-dependent modulation of mitochondrial function underlies some of nitrite’s protective actions. For example, nitrite-dependent post-translational modification of mitochondrial complex I is crucial for protection after ischemia/reperfusion and the nitrite-dependent stimulation of mitochondrial fusion regulates nitrite mediated preconditioning and adipocyte glucose uptake. Ongoing studies in the lab are investigating the mechanisms by which nitrite modulates mitochondrial efficiency as well as its regulation of mitochondrial kinase signaling.
4 – Myoglobin as a regulator of mitochondrial function.
The monomeric heme protein myoglobin, expressed in cardiac and skeletal muscle, has long been known to facilitate oxygen delivery to mitochondria in hypoxic conditions. However, recent studies have shown that myoglobin is expressed in tissues beyond the heart and muscle and has functions beyond oxygen binding and transport. For example, we showed that in hypoxia, myoglobin enzymatically reduces nitrite to nitric oxide, a potent inhibitor of mitochondrial respiration. Our lab is currently investigating how reactions of nitrite, nitric oxide and oxygen with myoglobin regulate mitochondrial function to modulate biological processes such as injury after ischemia/reperfusion, smooth muscle cell proliferation and cancer cell proliferation.
Journal Articles
Xu W, N Cardenes, C Corey, SC Erzurum and
S Shiva
. Platelets from asthmatic individuals show less reliance on glycolysis. PLoS One 10:e0132007, 2015.
Khoo NK, L Mo, S Zharikov, C Kamga-Pride, K Quesnelle, F Golin-Bisello, L Li, Y Wang and
S Shiva
. Nitrite augments glucose intake in adipocytes through the protein kinase A-dependent stimulation of mitochondrial fusion. Free Radic Biol Med 70:45-53, 2014.
Cardenes N, C Corey, L Geary, S Jain, S Zharikov, S Barge, EM Novelli and
S Shiva
. Platelet bioenergetic screen in sickle cell patients reveals mitochondrial complex V inhibition, which contributes to platelet activation. Blood 123:2864-2872, 2014.
Kamga Pride C, L Mo, K Quesnelle, RK Dagda, D Murillo, L Geary, C Corey, R Portella, S Zharikov, C St. Croix, S Maniar, CT Chu, NK Khoo and
S Shiva
. Nitrite activates protein kinase A in normoxia to mediate mitochondrial fusion and tolerance to ischaemia/reperfusion. Cardiovasc Res 101:57-68, 2014.
Zharikov S and
S Shiva
. Platelet mitochondrial function: From regulation of thrombosis to biomarker of disease. Biochem Soc Trans 41:118-123, 2013.
Shiva S
, Z Huang, R Grubina, LA Ringwood, PH MacArthur, W Xu, VM Darley-Usmar and MT Gladwin. Deoxymyoglobin is a nitrite reductase that generates NO and regulates mitochondrial function. Circ Res 100:654-661, 2007.
Shiva S
, X Wang, LA Ringwood, X Xu, S Yuditskaya, V Annavajjhala, H Miyajima, N Hogg, ZL Harris and MT Gladwin. Ceruloplasmin is a nitric oxide oxidase and nitrite synthase that determines endocrine NO homeostasis. Nat Chem Bio 2:486-493, 2006.
Sponsored Research
Regulation of oxidative stress and vascular remodeling by EBP50 - 7/10/2017 - 4/30/2021
NIH - R01 HL136282
Hemolysis-induced platelet mtROS drives TSP1 release to accelerate PH pathogenesis - 4/5/2017 - 1/31/2021
NIH - R01HL133003